Product Category

Reagent Grade

HEPES, Sodium Salt

Product Code: HEPN-5250 | Previously: HN5250

Intended For Use In Commercial Manufacturing, Diagnostic And Laboratory Applications

HEPES Sodium Salt is a zwitterionic salt which is used in the preparation of a HEPES buffer solution. Its pH range of 9.5-11.5 makes an ideal buffer for pH maintenance. HEPES Sodium Salt interferes with the Folin protein assay. The tendency of this buffer to form radicals makes it unsuitable for redox studies.

Product Specifications

Appearance and Color  
White / Crystals
Infrared Spectrum  
Conforms to Reference
Solubility (Turbidity) 0.1M aqueous Clear
Solubility (Color) 0.1M aqueous  Colorless
Solubility (Form) 0.1M aqueous Solution 
Water (by Karl Fischer)  ≤ 3.0%
Titration with H2SO4  ≥ 96.0%
Cytotoxicity  Pass
Enzymes DNase - Nickase
DNase – Endo / Exonuclease
RNase Detection
pH 1.0% aqueous)  
9.5 – 10.5
≤ 4.5 E.U./g
Yeast and Mold ≤ 100 CFU/g
Bioburden ≤ 100 CFU/g
Protease Detection  Pass
UV Absorbance 260nm 0.1M aqueous ≤ 0.04 Abs UN 
UV Absorbance 280nm 0.1M aqueous  ≤ 0.2 Abs UN
List of C of As files
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CAS #: 75277-39-3

Formula: C8H17N2NaO4S

Sol. In H2O (g/L): 990

F.W.: 260.29 g/mol

pH @ 20°C: 4.5 - 6.0

Useful pH: 7.8 - 8.2

pKa @ 20˚C: 7.5

General Product Description:

  • HEPES Sodium Salt is a White Crystalline product.
  • Molecular Formula: C8H17N2NaO4S
  • Molecular Weight: 260.29 g/mol.
  • CAS Number: 75277-39-3.
  • Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt.



Material represented by this grade is suitable to be used only as the following: Quality System Manufactured Process Chemical for use in further manufacturing or as a reagent chemical for commercial manufacturing, diagnostic and laboratory research. The material represented by this grade is not suitable to be used as an Excipient, Active Pharmaceutical Ingredient, Drug, Drug Product or household item.

Retest Date:

Two years from date of manufacture, testing and packaging.

Storage and Shipping Conditions:

Ship and Store in ambient temperature.

Package Sizes:

10kg, 25kg and 50kg pails.

Additional Packaging Information