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BioBuffer Solutions Brand Product
Reagent

HEPES

Enzyme Free

Product Code: HEPE-5201

Minimum Order Quantity: 100kg

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Product Code: HEPE-5201
Suitable for use in biological and biotech chemical process applications from R&D through scale production.

HEPES is a zwitterionic buffer used to maintain pH of media used in cell cultures. It is one of Good’s buffers that has a pKa value similar to its pH value, making it an ideal buffer for pH maintenance. A known limitation is its interference with the Folin protein assay. This buffer can form radicals, so it is not suitable for redox studies. HEPES is a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.

CAS #: 7365-45-9
Molecular Formula: C8H18N2O4S
F.W.: 238.31 g/mol

Product Specifications

Analysis Specifications
Absorbance (0.1M)

260nm

280nm

< = 0.0500 a.u.

< = 0.0500 a.u.

Appearance and Color White Crystals
Assay, Dried Basis > = 99.0%
Enzymes

DNase

RNase

Protease

None Detected

None Detected

None Detected

Loss on Drying < = 1.0%
pH (5%) 5.0 - 6.5
pKa Report from MFG CoA
Residue on Ignition < = 0.2%
Trace Metals

Arsenic (As)

Iron (Fe)

Lead (Pb)

< = 0.5 ppm

< = 5 ppm

< = 5 ppm

Key Product Features:

  • Appears as white crystals
  • Contains no known major food allergens (as defined by FDA and WHO)
  • The final product and its raw materials are not derived from nor come into contact with animal parts, animal products, and/or animal byproducts or derivatives.
  • Is not subject to genetic modification
  • Synonyms: N-(2-Hydroxyethyl) Piperazine-N'-2-Ethanesulfonic Acid; 4-(2-Hydroxyethyl) Piperazine-1-Ethanesulfonic Acid

Shelf-Life Policy:

Please inquire for information regarding shelf-life.

Storage & Shipping:

Refer to SDS.

Package Sizes:

1kg, 5kg, 10kg, 25kg, and 50kg

Bulk GMP Fine Chemicals exclusively manufactured in North America

American Manufactured - FDA Registered GMP Facilities: Parenteral Excipients, GMP Small Molecule Intermediates, GMP Biological Buffers, Parenteral Carbohydrates, GMP Chlorinated Amino Acids, GMP Solutions in Sterile Single Use Packaging and other Reagent & GMP Fine Chemicals.