HEPES, Reagent

BioBuffer Solutions Brand Product

Reagent

HEPES

Endotoxin Tested

Product Code: HEPE-5251

Minimum Order Quantity: 100kg
Lead Time: 2-4 Weeks

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Product Code: HEPE-5251

Suitable for use in biological and biotech chemical process applications from R&D through scale production.

HEPES is a zwitterionic buffer used to maintain pH of media used in cell cultures. It is one of Good’s buffers that has a pKa value similar to its pH value, making it an ideal buffer for pH maintenance. A known limitation is its interference with the Folin protein assay. This buffer can form radicals, so it is not suitable for redox studies. HEPES is a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.

CAS #: 7365-45-9
Formula: C₈H₁₈N₂O₄S
F.W.: 238.31 g/mol

Product Specifications

Analysis

Specifications

Absorbance (1M)

260 nm

280 nm

< = 0.1 a.u.

Appearance and Color

White Crystalline Powder

Assay, Dried Basis

> = 99.0%

Endotoxin (0.2% solution)

< = 0.10 EU/mL

Heavy Metals (Pb)

< = 5 ppm

Identification, IR

Conforms to Reference Standard

Iron (Fe)

< = 5 ppm

Loss on Drying

< = 1.0%

pH (5%)

5.0 - 6.5

pKa, 20°C

7.35 - 7.69

pKa, 25°C

7.2 - 7.8

Residue on Ignition

< = 0.2%

Solubility (1M)

Clear, Complete and Colorless

Sulfate

< = 500 ppm

Printable Version

Key Product Features:

Appears as a white crystalline powder
Contains no known major food allergens (as defined by FDA and WHO)
The final product and its raw materials are not derived from nor come into contact with animal parts, animal products, and/or animal byproducts or derivatives.
Is not subject to genetic modification
Synonyms: N-(2-Hydroxyethyl) Piperazine-N'-2-Ethanesulfonic Acid; 4-(2-Hydroxyethyl) Piperazine-1-Ethanesulfonic Acid

Shelf-Life Policy:

Please inquire for information regarding shelf-life.

Storage & Shipping:

Refer to SDS.

Package Sizes:

1kg, 5kg, 10kg, 25kg, and 50kg

Bulk GMP Fine Chemicals exclusively manufactured in North America

American Manufactured - FDA Registered GMP Facilities: Parenteral Excipients, GMP Small Molecule Intermediates, GMP Biological Buffers, Parenteral Carbohydrates, GMP Chlorinated Amino Acids, GMP Solutions in Sterile Single Use Packaging and other Reagent & GMP Fine Chemicals.