Product Category

Reagent Grade


Product Code: TRIS-5220 | Previously: TR5220

Intended Use Statement

Tris, commonly used as a buffer in biochemistry and molecular biology, stabilizes the pH of solutions in electrophoresis and other biological applications. It is also an enzyme inhibitor used in the biopharmaceutical industry and is commonly utilized in electro-chromatography, UV analysis and HPLC applications. Tris is extensively used as a biological buffer or a component of buffer solutions. Tris can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.

Product Specifications

Absorbance (40%) 290 nm 
0.02 a. u. max.
Appearance and Color  
White / Crystals
99.8%-100.1% min.
Heavy Metals  
5 ppm max.
Identification B  
Passes Test
Identification C  
Passes Test
Identification (IR)  
Passes Test
Insoluble Matter  

0.005% maximum

Karl Fischer Water  

2% maximum

Loss on Drying  

1% maximum

Melting Range  

168-172 oC

Residue on Ignition  
0.1% maximum 
Trace Metals Iron(Fe)
5 ppm maximum 
Printable Version
Key Compliance Attributes of Bio Ultra Grade
Suitable for Research and Diagnostic Application
C of A Available for Each Lot
Management of Change
Trace Metals Analyzed
Stability Testing Program
BioSpectra Supply Chain Audit Trail
Product Origin Statement
Customer Quality Audits
Customized Additional Specifications
Enzyme Tested
List of C of As files
No files available right now.

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COAs and Tech Docs


TRIS-5220 | TR5220

CAS #: 77-86-1

Formula: C4H11NO3

Sol. In H2O (g/L): 550

F.W.: 121.14 g/mol

pH @ 20°C: 10.0 - 11.5

Useful pH: 7.0 - 9.0

pKa @ 20˚C: 8.3

General Product Description:

  • Tromethamine is a White Crystalline product.
  • Molecular Formula: C4H11NO3
  • Molecular Weight: 121.14 g/mol.
  • CAS Number: 77-86-1.
  • Synonyms: Tris, Tromethamine, Tris (hydroxymethyl) aminomethane.

Intended Use Statement:

Material represented by this grade is suitable to be used only as the following: Quality System Manufactured Process Chemical for use in further manufacturing or as a reagent for laboratory and research. The material represented by this grade is not suitable to be used as an Excipient, Active Pharmaceutical Ingredient, Drug, Drug Product or household item.

Retest Date:

BioSpectra does not assign expiration or retest dates for Bio Ultra Grade materials.

Storage and Shipping Conditions:

Ship and Store in ambient temperature.

Package Sizes:

10 kg, 25 kg and 50 kg pails.

Additional Packaging Information