Reagent Grade
HEPES, Sodium Salt
Product Code: HEPN-5250 | Previously: HN5250
Intended For Use In Commercial Manufacturing, Diagnostic And Laboratory Applications
HEPES Sodium Salt is a zwitterionic salt which is used in the preparation of a HEPES buffer solution. Its pH range of 9.5-11.5 makes an ideal buffer for pH maintenance. HEPES Sodium Salt interferes with the Folin protein assay. The tendency of this buffer to form radicals makes it unsuitable for redox studies.
Product Specifications
ANALYSIS | SPECIFICATIONS | ||
---|---|---|---|
|
White / Crystals | ||
|
Conforms to Reference | ||
Solubility (Turbidity) 0.1M aqueous | Clear | ||
Solubility (Color) 0.1M aqueous | Colorless | ||
Solubility (Form) 0.1M aqueous | Solution | ||
Water (by Karl Fischer) | ≤ 3.0% | ||
Titration with H2SO4 | ≥ 96.0% | ||
Cytotoxicity | Pass | ||
|
Pass Pass Pass |
||
|
9.5 – 10.5 | ||
|
≤ 4.5 E.U./g | ||
Yeast and Mold | ≤ 100 CFU/g | ||
Bioburden | ≤ 100 CFU/g | ||
Protease Detection | Pass | ||
UV Absorbance 260nm 0.1M aqueous | ≤ 0.04 Abs UN | ||
UV Absorbance 280nm 0.1M aqueous | ≤ 0.2 Abs UN |
Key Compliance Attributes of Bio Ultra Grade |
---|
Suitable for Research and Diagnostic Application |
C of A Available for Each Lot |
BioSpectra Supply Chain Audit Trail |
Product Origin Statement |
Customer Quality Audits |
Customized Additional Specifications |
Enzyme Tested |
List of C of As files |
---|
No files available right now. |
COAs and Tech Docs
- Printable Spec Sheet
- Product Inquiry
- Compliance
- C of As
- SDS
- Test Methods
- Elemental Impurity Assessment


HEPN-5250
CAS #: 75277-39-3
Formula: C8H17N2NaO4S
Sol. In H2O (g/L): 990
F.W.: 260.29 g/mol
pH @ 20°C: 4.5 - 6.0
Useful pH: 7.8 - 8.2
pKa @ 20˚C: 7.5
General Product Description:
- HEPES Sodium Salt is a White Crystalline product.
- Molecular Formula: C8H17N2NaO4S
- Molecular Weight: 260.29 g/mol.
- CAS Number: 75277-39-3.
- Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt.
Compliance:
Material represented by this grade is suitable to be used only as the following: Quality System Manufactured Process Chemical for use in further manufacturing or as a reagent chemical for commercial manufacturing, diagnostic and laboratory research. The material represented by this grade is not suitable to be used as an Excipient, Active Pharmaceutical Ingredient, Drug, Drug Product or household item.
Retest Date:
Two years from date of manufacture, testing and packaging.
Storage and Shipping Conditions:
Ship and Store in ambient temperature.
Package Sizes:
10kg, 25kg and 50kg pails.
Additional Packaging Information
https://www.biospectra.us/technical/packaging