HEPES, Sodium Salt
BIO ULTRA GRADE | HN5250 | CAS#: 75277-39-3
Intended For Use in Commercial Manufacturing, Diagnostic and Laboratory Applications


BIO ULTRA GRADE | HN5250
CAS #: 75277-39-3
Molecular Formula:
C8H17N2NaO4S
Solubility in Water (g/L): 990
F.W.: 260.29 g/mol
pH @ 20°C: 4.5 - 6.0
Useful pH: 7.8 - 8.2
pKa @ 20˚C: 7.5
COAs and Tech Docs
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- C of As
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HEPES Sodium Salt is a zwitterionic salt which is used in the preparation of a HEPES buffer solution. Its pH range of 9.5-11.5 makes an ideal buffer for pH maintenance. HEPES Sodium Salt interferes with the Folin protein assay. The tendency of this buffer to form radicals makes it unsuitable for redox studies.
This is a non-GMP manufactured Product.
These are general specifications. BioSpectra will customize our products to meet your quality based requirements.
ANALYSIS | SPECIFICATIONS | ||
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White / Crystals | ||
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Conforms to Reference | ||
Solubility (Turbidity) 0.1M aqueous | Clear | ||
Solubility (Color) 0.1M aqueous | Colorless | ||
Solubility (Form) 0.1M aqueous | Solution | ||
Water (by Karl Fischer) | ≤ 3.0% | ||
Titration with H2SO4 | ≥ 96.0% | ||
Cytotoxicity | Pass | ||
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Pass Pass Pass |
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9.5 – 10.5 | ||
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≤ 4.5 E.U./g | ||
Yeast and Mold | ≤ 100 CFU/g | ||
Bioburden | ≤ 100 CFU/g | ||
Protease Detection | Pass | ||
UV Absorbance 260nm 0.1M aqueous | ≤ 0.04 Abs UN | ||
UV Absorbance 280nm 0.1M aqueous | ≤ 0.2 Abs UN |
Key Compliance Attributes of Bio Ultra Grade |
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Suitable for Research and Diagnostic Application |
C of A Available for Each Lot |
Management of Change |
Trace Metals Analyzed |
Stability Testing Program |
BioSpectra Supply Chain Audit Trail |
Product Origin Statement |
Customer Quality Audits |
Customized Additional Specifications |
Enzyme Tested |
List of C of As files |
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No files available right now. |
General Product Description:
• HEPES Sodium Salt is a White Crystalline product.
• Molecular Formula: C8H17N2NaO4S
• Molecular Weight: 260.29 g/mol.
• CAS Number: 75277-39-3.
• Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt.
Compliance:
Material represented by this grade is suitable to be used only as the following: Quality System Manufactured Process Chemical for use in further manufacturing or as a reagent chemical for commercial manufacturing, diagnostic and laboratory research. The material represented by this grade is not suitable to be used as an Excipient, Active Pharmaceutical Ingredient, Drug, Drug Product or household item.
Expiration/Retest Date:
Two years from date of manufacture, testing and packaging.
Storage and Shipping Conditions:
Ship and Store in ambient temperature.
Package Sizes:
10kg, 25kg and 50kg pails.