HEPES, CAS# 7365-45-9, Zwitterionic Buffer, Good's Buffer

HEPES, Free Acid

Molecular Fomula: C₈H₁₈N₂O₄S
F.W.: 283.3
CAS#: 7365-45-9
pH @ 20° C: 5.6 - 6.5
Useful pH: 6.8 - 8.2
pKa @ 20° C: 7.5
Solubility in water (g/ml): 0.4


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Description:
HEPES is a Zwitterionic Buffer used to maintain pH of media used in cell cultures. It is one of Good's twelve buffers that has a pKa value similar to its pH value making it an ideal buffer for pH maintenance. It has only one restriction, it interferes with the Folin protein assay. This buffer can form radicals so it is not suitable for redox studies.

Specifications Product Links

HEPES Free Acid

(N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid)
C₈H₁₈N₂O₄S ^ F.W. 283.3 g/mol ^ CAS# 7365-45-9

ANALYSIS		SPECIFICATIONS

		BioUltra HEP-13	BioSynthesized HEP-12	Bio Reagent HEP-11

Appearance and Color		White/Crystal	White/Crystal	White to Off-White/Crystal
Assay		99.5% min.	99.0% min.	98.0% min.
Chloride		0.005% max.
Heavy Metals		0.0001% max.
Insoluble Matter		0.01% max.
Identification (IR)		Passes Test	Passes Test	Passes Test
Loss on Drying		0.5% max.
pH 5% Solution		5.0 - 6.5	5.0 - 6.5
pKa		7.45 - 7.65
Residue on Ignition		0.1% max.	0.2% max.
Solubility		Passes Test	Passes Test
Sulfate		0.005% max.
Absorbance	@ 280	0.050 a.u. max.	0.090 a.u. max.
	@ 260	0.050 a.u.max.	0.060 a.u. max.
	@ 250	0.080 a.u. max.	0.060 a.u. max.
Trace Metals	Arsenic (As)	0.0005% max.
	Copper (Cu)	0.0005 max.
	Iron (Fe)	0.0005 max.
	Lead (Pb)	0.0005 max.
Enzymes	RNase	None Detected
	DNase	None Detected
	Protease	None Detected

HEPES MSDS (CAS# 7365-45-9)

Merck Merck 13,4672

Beilstein Beil. 23,V,2,379

Product	Molecular	CAS #	Details
MES, Monohydrate	C₆H₁₃NO₄S H₂O	145224-94-8
MOPS, Free Acid	C₇H₁₅NO₄S	1132-61-2
MOPS, Free Acid	C₇H₁₅NO₄S	1132-61-2

Bio Reagent (Good)
Quality tested from approved suppliers of BBS for Industry-Standard use.

Bio Synthesized (Better)
Manufactured in a quality system based facility offering traceability and consistent supply.

Bio Ultra (Best)
Manufactured at BBS to meet the highest quality and regulatory standards.