Tris, Tromethamine, CAS# 77-86-1

Tris / Tromethamine

Molecular Fomula: NH₂C(CH₂OH)₃
F.W.: 121.14
CAS#: 77-86-1
pH @ 20° C: 10.0 - 11.5
Useful pH: 7.0 - 9.0
pKa @ 20° C: 8.0
Solubility in water (g/ml): 0.65


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Description:
Tris, commonly used as a buffer in biochemistry and molecular biology, stabilizes the pH of solutions in electrophoresis or other biological applications. It is also an enzyme inhibitor for use in the biopharmaceutical industry and is commonly utilized in electro chromatography, UV analysis and HPLC applications.

Specifications Product Links

Tris / Tromethamine

NH₂C(CH₂OH)₃ ^ F.W. 121.14 g/mol ^ CAS# 77-86-1

ANALYSIS		SPECIFICATIONS

		Bio Ultra TRS-13 Meets FCC, USP, ACS	Bio Synthesized TRS-12 Meets FCC, USP, ACS	Bio Reagent TRS-11

Absorbance (40%) @ 290		0.2 a.u. max.	0.2 a.u. max.
Appearance and Color		White/Crystals	White/Crystals	White to Off-White Crystals
Assay		99.8-100.1%	99.8-100.1%.	98.0% min.
USP Heavy Metals		0.0005% max.	0.0005% max.
Identification (IR)		Passes Test	Passes Test	Passes Test
Identification B		Passes Test	Passes Test
Identification C		Passes Test	Passes Test
Insoluble Matter		0.005% max.	0.005% max.
Karl Fisher Water		2% max.	2% max.
Loss on Drying		1.0% max.	1.0% max.
Melting Range °C		168°C - 172°C	168°C - 172°C
pH 5% Solution		10.0 - 11.5	10.0 - 11.5
Residue on Ignition		0.1% max.	0.1% max.
Trace Metals	Arsenic (As)	0.0005% max.
	Calcium (Ca)	0.0005% max.
	Copper (Cu)	0.0005% max.
	Iron (Fe)	0.0005% max.	0.0005% max.
	Lead (Pb)	0.0005% max.
	Magnesium (Mg)	0.0005% max.
Enzymes	RNase	None Detected
	DNase	None Detected
	Protease	None Detected

Tris MSDS (CAS# 77-86-1)

Beilstein Beil. 4,IV,1903

Merck Merck 13,9842

"Dissociation constants and related thermodynamic quantities of the protonated acid form of tris-(hydroxymethyl)-aminomethane in mixtures of 2-methoxyethanol and water at different temperatures". J. Electroanal. Chem. 162 (1-2): 285–305. doi:10.1016/S0022-0728(84)80171-0.

Gomori, G., Preparation of Buffers for Use in Enzyme Studies. Methods Enzymology., 1, 138-146 (1955).

Product	Molecular	CAS #	Details
MES, Monohydrate	C₆H₁₃NO₄S H₂O	145224-94-8
MOPS, Free Acid	C₇H₁₅NO₄S	1132-61-2
MOPS, Free Acid	C₇H₁₅NO₄S	1132-61-2


MOPS, Free Acid

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