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Guanidine Hydrochloride

Chemical Properties:
CAS Number: 50-01-1
F.W.: 95.53 g/mol
Molecular Formula: NH₂(NH)NH₂●HCl

Industry Uses:
Guanidine hydrochloride is widely used in the purification of proteins and nucleic acids. It is a powerful protein-denaturing agent used in the isolation of RNA from cell extracts. RNase activity is inhibited in the presence of Guanidine Hydrochloride.

Test	Specifications
	Type 24	Type 23	Type 22	Type 21
Appearance and Color	White/Crystals	White/Crystals	White/Crystals	White/Crystals

Assay, Dried Basis	99.5-101.0%	99.5% min.	99.5-101.0%	98.0% min.
Acidity (HCl)	0.01% max.	0.01% max.
Melting Range	184°-188°C	184°-188°C	176°-188°C	176°-188°C
LOD	0.5% max.	0.5% max.	0.5% max.
Absorbance (6M)	0.03@275nm max.	0.03@260nm max.	Report @ 270nm	0.06@300nm max.
	0.03@260nm max.		Report @ 260nm
	0.20@230nm max.
Copper (Cu)	0.00002% max.	0.001% max.	Report
Iron (Fe)	0.0002% max.	0.001% max.	0.0002% max.
Lead (Pb)	0.00005% max.	0.001% max.	Report
Arsenic (As)	0.00005% max.	0.001% max.	Report
Identity (FTIR)				Matches
Sulfate	0.005% max.
Nitrate	0.005% max.
Cyanide	0.1ppm max.
HM		0.001% max.
Water Insolubles	0.3% max.	0.3% max.	0.3% max.	0.3% max.
Melamine & Related	Report	Report	Report
ROI	0.005% max	0.2% max.
RNase	None Detected	None Detected
DNase	None Detected	None Detected
Protease	None Detected	None Detected