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Tris HCl

 

Chemical Properties:
CAS Number:  1185-53-1
F.W.:  157.60 g/mol
Molecular Formula:  NH2C(CH2OH)3·HCl

Industry Uses:
Tris hydrochloride is a buffer component in molecular biology, tissue culture, and electrophoreses methods. It can be used as an alternative to or in combination with Tris(hydroxymethyl)aminomethane in many buffers.


Test

Specifications


Type 49

Type 48

Appearance and Color

White/Crystals

White/Crystals

Assay

99.0% - 103.0%

98.0% min.

Assay Dried Basis

99.0% min.


Identity IR

To Pass Test

To Pass Test

Absorbance (1M)

0.01 max. @ 400 nm


0.06 max. @ 280 nm

0.10 max. @ 280 nm

0.06 max. @ 260 nm


Elemental

Arsenic (As)

0.0001% max.


Calcium (Ca)

0.0001% max.


Copper (Cu)

0.0001% max.


Iron (Fe)

0.0001% max.


Magnesium (Mg)

0.0001% max.


Lead (Pb)

0.0001% max.


pH (0.5M)

3.5-5.0

3.5-5.0

Melting Range

147-153°C

147-153°C

Loss on Drying (105°C)

0.5% max.

1.0% max.

Enzymes

DNase

None Detected


RNase

None Detected


Protease

None Detected