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Tris / Tromethamine

 

Chemical Properties:
CAS Number:  77-86-1
F.W.:  121.14 g/mol
Molecular Formula:  NH2C(CH2OH)3

Industry Uses:
Tris is used in enzyme purification and assay protocols. It is a buffer component in molecular biology, tissue culture and electrophoresis methods. Tris is suitable for enzyme digestions of DNA.

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Specifications


Type 45

Type 43

ACS Type 44

Appearance and Color

White/Crystals

White/Crystals

White/Crystals

Insoluble Matter

0.005%  max.

  

0.005%  max.

Assay, Dried Basis

99.9% min.

  

99.8-100.1%

Assay

99.0-101.0%

99.0-101.0%

 

Melting Range

168°-172°C

168°-172°C

 

Identity

To Pass Test

To Pass Test

 

Absorbance (1M)

0.01@400nm  max.

 

 
 

0.01@280nm  max.

 

 
 

0.06@260nm  max.

 

 

Absorbance (40% solution)

 

 

0.02 max.@400nm
0.2 max. @290nm
0.2 max. @280nm

Loss on Drying

 

1.0%  max

1.0%  max
Water (Karl Fischer)

 

 

2% max
Arsenic  (As)

0.0001%  max.

 

 
Calcium (Ca)

0.0001%  max.

 

 
Copper (Cu)

0.0001%  max.

 

 
Iron (Fe)

0.0001%  max.

 

0.0005% max.
Magnesium (Mg)

0.0001%  max.

 

 
Lead (Pb)

0.0001%  max.

0.001%  max.

 
USP Heavy Metals

0.001%  max.

0.001%  max.

0.0005% max.
pH  5% Solution

10.0-11.5

10.0-11.5

 
pH (0.1M solution)

10.0-11.0

 

10.0-11.0
pH(0.05M solution)

 

 

10.3-10.5
Residue On Ignition

0.1%  max.

0.1%  max.

 

 

 
Enzymes

RNase

None Detected

 

 

DNase

None Detected

 

 

Protease

None Detected